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NBBC Guide

5 Overview

The output of non-B burden in NBBC is a matrix that contains a list of genes and a list of non-B types. The data in the matrix represents the user-selected non-B burden calculation chosen from among the multiple normalizations offered by the web server.

The goal of the “Gene Screen” module in NBBC is to conduct gene-level analyses of non-B representation that can be helpful in focusing on a single or subset of genes of interest for hypothesis generation.

This module allows users to explore the non-B burden distribution of each non-B type among the query genes, the total (cumulative) non-B burden for each gene in the query, the composition of non-B burden representations at the gene level, and heatmap clustering of non-B burden among non-B types and genes.

To achieve this goal, NBBC visually dissects non-B burden into four components:

  1. Burden distribution of each non-B type among the query genes

  2. Total (cumulative) non-B burden for each gene in the query

  3. Composition of non-B burden representations at the gene level

  4. Heatmap clustering of non-B burden among non-B types and genes.

These components provide users with a comprehensive and detailed understanding of non-B burden at the gene level and facilitate focused hypothesis generation for further exploration.